Serotype and Virulence Gene of E.coli and Associated with Rota Virus Isolated from Patients with Diarrhea InfectionAuthor(s): Zahrah Adnan Al-Shammarri*
Diarrheal infection are a sever public health problem and a major causes of mortality of children . E.coli is one the most important microbial agent of diarrhea. Rotavirus is the most common causes of sever gastroenteritis and diarrhea in infant and young children in world. Seventy-five diarrheal samples was isolated from children have sign and symptoms as diarrhea, vomiting abdominal pain and dehydration. Samples were isolated from Al- sadder hospital/pediatric department from January to May 2020. In material and methods was been used bacteriological culture media, biochemical test, Gram stain and Vitek-2 to detect E.coli in 37C° for 24 hr . For detection of Rota virus use Enzyme linked immunosorbent assay. Cav C gene and Tra t gene are a virulence factors gene in E.coli were detected by DNA extraction and Conventional PCR .In the results age groups and sex distribution with diarrheal infection male was 41 (54.7%) and female 34 (45.3%) regarding to sex distribution with E.coli and Rota virus male is more effective with E.coli 17 (41.5%) and female 20 (58.8%) but Rota virus associated with diarrheal and male was recorded 12 (29.3%) and female (11.82%). E.coli was enrolled 37 (49.34%), Rota virus 23 (30.7%) and mix infection 16 (20%) Based on types of feeding bottle feeding was recorded a high rate (40%) which causes diseases. A virulence (gene tra T and cva C) gene in E.coli was detected.