Impact of DNA Repair System Genes RAD-18 and XRCC1 Polymorphism in Depression Disorders PatientsAuthor(s): Mona N Al-Terehi*, Zahraa Haleem AlQaim and Arafat Hussein Aldujaili
The Depressive Disorders (DDs) it is one of the most widespread psychiatric pathology forms and the relation of DNA repair with DD still under investigation, the present study was conducted to detect the RAD-18 Arg302Gln (rs373572) and XRCC1 Arg399Gln (28152) G>A gene polymorphisms in the DD patients, allele specific PCR and PCR-CTTP were used, the results show the age of patients (38.10 ± 12.74) year with BMI (24.98 ± 3.96) kg/m2 have duration of disease (7.50 ± 7.70) years and of control was (38.36 ± 12.85) year with BMI (27.02 ± 4.44) Kg/m2, non-significant differences of family history and significant variations were observed to dietary and exercise in study groups. The genotyping The RAD-18 show two alleles (A and G) and two genotyping (AA and GG), the AA was found in all patients and in 92% of the control group, GG found in 8% of the control group and didn’t find in patients in non-significant differences (Od 0.2410, CI% 0.0109- 5.3247, P 0.36), there was non-significant differences in compare GA and GG genotyping (Od 17.000, CI% 0.13- 2166.90, P 0.25). the allele observed in patients (1) but G didn’t appear , in control group A was 0.92% in control and G found in 0.08. There were non- significant differences of allele frequency (Od 0.2410, CI% 0.010 -5.324, P 0.367). The XRCC1 genotyping show two alleles (A and G) and three genotyping (AA, GG and AG), the GG was less frequent in patients than the control group (26.31%, 44%), GA was more frequent in patients group (73.68%) than control group (48%) in non-significant differences (Od 2.2000 CI% 0.605- 7.999, P 0.231), AA observed in 8% of control group but didn't observe in patients group in non-significant differences (Od 5.8000, CI% 0.253-132.56, P 0.270), finally the alleles frequency show that G allele observed in 0.63 and 0.68 in patients and control group respectively also 0.36 and 0.32 of A allele in non-significant differences (Od 1.2396, CI% 0.3534-4.3484, P 0.737). the present study concluded that no-association between repair genes RAD-18 and XRCC1 with DD disease.