Impact of Apurinic/Apyrimidinic Endonuclease Asp148Glu (rs3136820) Gene Polymorphism and APE1 Level in Depression Disorders PatientsAuthor(s): Mona N. Al-Terehi, Zahraa F. AL-Khero, Abeer Ameen Baqer, Mohammed Abed Jawad*, Safa K. Hachim, Samah Sajad Kadim, Ali Ahmed Nayyef and Sarah Hussein Ali Alallo
The repair system processes are very important in the maintenance of genome against mutagenic factors, the present study deal with Apurinic/apyrimidinic endonuclease gene polymorphism and its level in depression disorder patients, blood samples were collected from patients and control to serum separation for APE1 detection and DNA extraction for APE1 Asp148Glu (rs3136820) gene polymorphism by CTTP-PCR technique, finding of present research show that the mean age of patients and control were non-significant differences (P 0.156), the BMI was significant differences (P 0.042) between groups. non- significant decrement was appeared in the APE1 level in the patients group (P 0.183), the CTTP-PCR products show two alleles (T and G) and three genotyping in addition to deletion mutation (TT, GT and GG), significant differences was observed in TG allele that low frequent in patients than the control group (OR 5.0909, CI% 1.3190-19.649, P 0.0182). The GG allele shows high frequent in patients than the control group in non-significant differences (OR 3.8500, CI 95% 0.7614-19.468, P 0.103). Deletion mutation also found in non-significant differences (OR 1.7143 CI% 0.1305-22.5139, P 0.6816). The effect of APE1 genotyping in APE1 level show non-significant differences in APE1 levels, according to APE1 genotyping (P 0.870) although of decreasing the level of APE1 in TG in patients than other genotyping. There was a weak relation of APE1 levels, and no association between APE1 Asp148Glu (rs3136820) genotyping with depression disorder.